Fig 2: A schematic diagram illustrating the involvement of ATXN8OS and miR-204 in breast cancer. lncRNA, long non-coding RNA; ATXN8OS, ataxin 8 opposite strand; miR-204, microRNA-204; JAK2, tyrosine protein kinase JAK2; FOXA1, forkhead box A1; ANGPT1, angiopoietin-1; TGFβR2, TGF-β receptor type 2; ORF, open reading frame; UTR, untranslated region.

Fig 3: Telaprevir antiproliferative effect is correlated to the ratio of FOXA1/IGF1‐R expression. (A) Bar graph of telaprevir (Tel) inhibition concentration 50 (IC50‐μm) value measured in the indicated cancer cell lines at 5 days. Data are the means ± the SD n = 3. (B) Western blotting analyses of FOXA1, IGF1‐R and vinculin protein levels in the indicated cancer cell lines. This blot has been performed twice. (C) The scatter plot of Spearman correlation analyses for the ratio of FOXA1/IGF1‐R protein levels and Tel IC50 (μm) on the proliferation of the above‐mentioned cancer cell lines. [Colour figure can be viewed at wileyonlinelibrary.com]

Fig 4: Analysis of telaprevir‐induced apoptosis. (A) Western blotting and (A’) relative densitometric analyses of cleaved PARP and tubulin (Tub) in MCF‐7 cells treated with different doses of telaprevir (Tel 10–40 μm) for 48 h and staurosporine (STS 100 nm) for 24 h. Data are the mean ± standard deviation with P‐value < 0.0001. n = 4 for all samples but for 40 μm where n = 3. **** indicates significant differences with respect to the control sample (0) calculated with one‐way ANOVA followed by the Tukey post‐test. Western blotting analyses of telaprevir (Tel) effect on cleaved PARP, FOXA1 and tubulin (Tub) levels in the presence of FOXA1 siRNA (B) and NVP (C). Cells were transfected with FOXA1 siRNA and treated with 1 μm NVP before 48 h of 20 μm Tel treatment. (D) Western blotting analyses of cleaved PARP, FOXA1 and vinculin expression in MCF‐7 cells pretreated with different doses of IGF (10–250 ng·mL−1) for 1 h before 48 h of 20 μm Tel treatment. Densitometric analyses of panel (B) (C) and (D) are shown in Fig. S8C–H. n = 4 for (B) (C) and (D), n = 3 for FOXA1 levels in panel D. (E–H) Western blotting analyses of cleaved PARP and tubulin in HeLa, SKOV3, MCF‐7, and SKBR3 cells treated with telaprevir (Tel 20 μm) for 48 h. Densitometric analysis are shown in Fig. S9B. n = 3 for all the cell lines and n = 4 for SKOV3 cells.

Fig 5: Schematic illustration of BARX2/FOXA1/HK2 axis promotes LUAD progression and energy metabolism reprogramming. LUAD, lung adenocarcinoma.